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DEMONSTRATED METHODS

This workshop will include hands-on, training demonstrations of three advanced techniques for measuring denitrification in aquatic and terrestrial systems: membrane inlet mass spectrometry, gas-flow soil core with N2 chromatography, and isotope pairing.  We anticipate the development of an online training module for each method prior to the workshop.  Below are pertinent references for each technique.  Copyright restrictions prevent the posting of full-text publications on this site.  However, where available, links to papers posted on authors' personal or institutional websites are provided.

N2/Ar Membrane Inlet Mass Spectrometry (MIMS)

1. Kana, T. M., C. Darkangelo, M. D. Hunt, J. B. Oldham, G. E. Bennett, and J. C. Cornwell. 1994. A membrane inlet mass spectrometer for rapid high precision determination of N2, O2, and Ar in environmental water samples. Analytical Chemistry 66: 4166-4170. [Full Text]
2. Kana, T. M., M. B. Sullivan, J. C. Cornwell and K. Groszkowski. 1998. Denitrification in estuarine sediments determined by membrane inlet mass spectrometry. Limnology and Oceanography 43:334-339. [Full Text]
3. Cornwell, J. C., W. M. Kemp, and T. M. Kana. 1999. Denitrification in coastal ecosystems: methods, environmental controls, and ecosystem level controls, a review. Aquatic Ecology 33:41-54. [Publisher]
4. Kana, T. M., J. C. Cornwell, L. Zhong. 2006. Determination of denitrification in the Chesapeake Bay from measurements of N2 accumulation in bottom water. Estuaries and Coasts 29:222-231. [Publisher] [Author]

Gas-flow soil core with N2 chromatography

1. Butterbach-Bahl, K., G. Willibald and H. Papen. 2002. Soil core method for direct simultaneous determination of N2 and N2O emissions from forest soils. Plant and Soil 240:105-116. [Publisher]

Isotope pairing technique

1. Nielsen, L. P. 1992. Denitrification in sediment determined from nitrogen isotope pairing. FEMS Microbiology Letters 86:357-362. [Publisher]
2. Steingruber, S. M., J. Friedrich, R. Gächter, and B. Wehrli. 2001. Measurement of denitrification in sediments with the 15N isotope pairing technique. Applied and Environmental Microbiology 67:3771-3778. [PubMed Central Full Text]
3. Dalsgaard, T. (ed.), L. P. Nielsen, V. Brotas, P. Viaroli, G. Underwood, D.B. Nedwell, K. Sundbäck, S. Rysgaard, A. Miles, M. Bartoli, L. Dong, D.C.O. Thornton, L.D.M. Ottosen, G. Castaldelli, and N. Risgaard-Petersen. 2000: Protocol handbook for NICE – Nitrogen Cycling in Estuaries: A project under the EU research programme: Marine Science and Technology (MAST III). National Environmental Research Institute, Silkeborg, Denmark. 62 pp. [NICE Project] [Handbook]